Mycotoxins

Introduction

Items

1. Aflatoxin
(Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2)
Summary
1) Simultaneous analysis of mycotoxins by liquid chromatography/ tandem mass spectrometry
2) Simultaneous analysis of aflatoxins by liquid chromatography
3) Simultaneous analysis of aflatoxins by liquid chromatography-photochemical reactor
4) Fluorescence densitometry
4)-1 Peanut meal
4)-2 Cottonseed meal
4)-3 Corn
4)-4 Formula feeds

[Other analysis methods］
1) Screening method
2) Quantification of aflatoxin B1 by international standard (ISO 14718(1998))
3) Aflatoxin B1 test in grains, beans, nuts and seeds, and spices [Notification No. 0728003 dated July 28, 2008 from the Inspection and Safety Division, Department of Food Safety, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare]
4) Fluorescence densitometry

2. Sterigmatocystin
Summary
1) Simultaneous analysis of mycotoxins by liquid chromatography/ tandem mass spectrometry
2) Liquid chromatography

3. Zearalenone
Summary
1) Simultaneous analysis of mycotoxins by liquid chromatography/ tandem mass spectrometry
2) One-component analysis method by liquid chromatography/mass spectrometry
3) Liquid chromatography
3)-1 Feeds
3)-2 Grains
3)-3 Formula Feeds

[Other analysis methods］
1) ELISA method

4. Trichothecene mycotoxins
Summary
a) HT-2 toxin
b) T-2 toxin
c) Neosolaniol
d) Fusarenon-X
e) 3-Acetyldeoxynivalenol, 15-Acetyldeoxynivalenol
f) Deoxynivalenol, Nivalenol

5. Fumonisin
(Fumonisin B1, Fumonisin B2, Fumonisin B3)
Summary
1) Simultaneous analysis of fumonisin by liquid chromatography/ mass spectrometry
2) Simultaneous analysis of fumonisin B1 and B2 by liquid chromatography

[Other analysis methods］
1) ELISA method

6. Ochratoxin A
Summary
1) Liquid chromatography (1)
2) Simultaneous analysis of ochratoxin A and citrinin by liquid chromatography
3) Liquid chromatography (2)

[Other analysis methods］
1) ELISA method

7. Citrinin
Summary
1) Simultaneous analysis of ochratoxin A and citrinin by liquid chromatography

8. Monographs of endophyte toxins
Summary
8-1 Ergovaline
1) Liquid chromatography
8-2 Lolitrem B
1) Liquid chromatography
8-3 (Reference)Endophyte hypha
1) Rose bengal staining and aniline blue staining

List of Mycotoxin Analysis Methods

List of Mycotoxin Analysis Methods

  Simultaneous analysis of mycotoxins One-Conponent analysis
Simultaneous analysis of AF Simultaneous analysis of AF1 Simultaneous analysis of DON/NIV/T2 Simultaneous analysis of trichotecene mycotoxins Simultaneous analysis of trichothecene mycotoxins Simultaneous analysis of DON/NIV Simultaneous analysis of fumonishin Simultaneous analysis of fumonishin1 Simultaneous analysis of ochratoxin A and citrinin
Aflatoxin B1 ● ○ ○ ○            
B2 － ○ ○ ○            
G1 － ○ ○ ○            
G2 － ○ ○ ○            
Sterigmatocystin ● ○                
Zearalenone ● ○                
HT-2 toxin ●                  
T-2 toxin ● ○     ○          
Neosolaniol － ○                
Fusarenon-X － ○       ○        
3-Acetyldeoxynivalenol －         ○        
15-Acetyldeoxynivalenol －         ○        
Deoxynivalenol － ○     ○ ○ ○      
Nivalenol － ○     ○ ○ ○      
Fumonisin B1 －             ○ ○  
B2 －             ○ ○  
B3 －             ○    
Ochratoxin A ●                 ○
Citrinin －                 ○
Ergovaline ●                  
Lolitrem B ●                  

Note1)● indicates a component which has an one-component analysis method; ○ indicates components that can be analyzed simultaneously by a simultaneous analysis method.
2) DON, deoxynivalenol; NIV, nivalenol.

List of Analysis Methods for Mycotoxin

  TLC GC LC LC-MS LC-MS/MS Minicolumn ELISA
Aflatoxin B1 ○   ○   ○ △ △
Aflatoxin (total)     ○   ○   △
Sterigmatocystin     ○   ○    
Zearalenone     ○ ○ ○   △
HT-2 toxin       ○      
T-2 toxin   ○   ○ ○   △
Neosolaniol         ○    
Fusarenon-X         ○    
3-Acetyldeoxynivalenol ○            
15-acetyldeoxynivalenol ○            
Deoxynivalenol   ○ ○ ○ ○   △
Nivalenol   ○ ○ ○ ○    
Fumonisin     ○ ○     △
Ochratoxin A     ○       △
Citrinin     ○        

○: Method listed in the Feed Analysis Standards
△: Other method

List of Hazards in Feed Materials

Feed Materials Mycotoxin
Aflatoxin Ochratoxin Zearalenone Deoxynivalenol Nivalenol Fumonisin T-2 Toxin
Main Materials Corn ○   ○ ○ ○ ○ ○
Milo ○ ○ ○ ○ ○ ○ ○
Grain Rye   ○   ○ ○ ○  
Oats   ○          
Lupine              
Wheat   ○   ○ ○    
Barley   ○ ○ ○ ○ ○  
Vegetable Protein Feed Soybean Meal ○            
Rapeseed Meal              
Corn Gluten Meal     ○     ○  
Peanut Meal ○            
Palm Meal ○            
Sesami Mieal ○            
Pineapple Meal ○            
Animal Protein Feed Fish Meal              
Meat and Bone Meal              
Brans Bran   ○   ○ ○ ○  
Corn Gluten Feed     ○ ○   ○ ○
Rice Bran              
Hominy Feed              
Corn Germ Meal              
Brewer's Pellet              
Tapioca Pellet              
Barley Bran           ○  
Roughage Feed Alfalfa Pellet              
Hay Cube              
Beet Pulp              
Hay              
Cottonseed ○            
Nonfat Dry Milk              
Molasses              
Others Cassava ○            
Screening Pellets   ○   ○ ○    
Corncob Meal           ○  

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